High throughput sequencing reveals novel and abiotic stress-regulated microRNAs in the inflorescences of rice.

ABSTRACT:

Background

MicroRNAs (miRNAs) are small RNA molecules that play important regulatory roles in plant development and stress responses. Identification of stress-regulated miRNAs is crucial for understanding how plants respond to environmental stimuli. Abiotic stresses are one of the major factors that limit crop growth and yield. Whereas abiotic stress-regulated miRNAs have been identified in vegetative tissues in several plants, they are not well studied in reproductive tissues such as inflorescences.

Results

We used Illumina deep sequencing technology to sequence four small RNA libraries that were constructed from the inflorescences of rice plants that were grown under control condition and drought, cold, or salt stress. We identified 227 miRNAs that belong to 127 families,

SUBMITTER: Barrera-Figueroa BE 

PROVIDER: S-EPMC3431262 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature