Quantification of the internalization patterns of superparamagnetic iron oxide nanoparticles with opposite charge.

ABSTRACT: Time-resolved quantitative colocalization analysis is a method based on confocal fluorescence microscopy allowing for a sophisticated characterization of nanomaterials with respect to their intracellular trafficking. This technique was applied to relate the internalization patterns of nanoparticles i.e. superparamagnetic iron oxide nanoparticles with distinct physicochemical characteristics with their uptake mechanism, rate and intracellular fate.The physicochemical characterization of the nanoparticles showed particles of approximately the same size and shape as well as similar magnetic properties, only differing in charge due to different surface coatings. Incubation of the cells with both nanoparticles resulted in strong differences in the internalization rate and in the intracellular l

SUBMITTER: Schweiger C 

PROVIDER: S-EPMC3431280 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature