Systematic CpG islands methylation profiling of genes in the wnt pathway in epithelial ovarian cancer identifies biomarkers of progression-free survival.

ABSTRACT:

Purpose

Wnt pathways control key biological processes that potentially impact on tumor progression and patient survival. We aimed to evaluate DNA methylation at promoter CpG islands (CGI) of Wnt pathway genes in ovarian tumors at presentation and identify biomarkers of patient progression-free survival (PFS).

Experimental design

Epithelial ovarian tumors (screening study n = 120, validation study n = 61), prospectively collected through a cohort study, were analyzed by differential methylation hybridization at 302 loci spanning 189 promoter CGIs at 137 genes in Wnt pathways. The association of methylation and PFS was examined by Cox proportional hazards model.

Results

DNA methylation is associated with PFS at 20 of 302 loci (P < 0.05, n = 111), with 5 loci significant

SUBMITTER: Dai W 

PROVIDER: S-EPMC3431504 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature