Insights into processing and cyclization events associated with biosynthesis of the cyclic Peptide kalata B1.

ABSTRACT: Plant cyclotides are the largest family of gene-encoded cyclic proteins. They act as host defense molecules to protect plants and are promising candidates as insecticidal and nematocidal agents in agriculture. For this promise to be realized a greater understanding of the post-translational processing of these proteins is needed. Cyclotides are cleaved from precursor proteins with subsequent ligation of the N and C termini to form a continuous peptide backbone. This cyclization step is inefficient in transgenic plants and our work aims to shed light on the specificity requirements at the excision sites for cyclic peptide production. Using the prototypic cyclotide kalata B1 (kB1) expressed from the Oak1 gene, MALDI-TOF mass spectrometry was used to examine the cyclization efficiency when mu

SUBMITTER: Conlan BF 

PROVIDER: S-EPMC3431668 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature