Expression, purification and characterization of enoyl-ACP reductase II, FabK, from Porphyromonas gingivalis.

ABSTRACT: The rapid rise in bacterial drug resistance coupled with the low number of novel antimicrobial compounds in the discovery pipeline has led to a critical situation requiring the expedient discovery and characterization of new antimicrobial drug targets. Enzymes in the bacterial fatty acid synthesis pathway, FAS-II, are distinct from their mammalian counterparts, FAS-I, in terms of both structure and mechanism. As such, they represent attractive targets for the design of novel antimicrobial compounds. Enoyl-acyl carrier protein reductase II, FabK, is a key, rate-limiting enzyme in the FAS-II pathway for several bacterial pathogens. The organism, Porphyromonas gingivalis, is a causative agent of chronic periodontitis that affects up to 25% of the US population and incurs a high national burde

SUBMITTER: Hevener KE 

PROVIDER: S-EPMC3432161 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature