Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization and adipogenic differentiation.

ABSTRACT: Isolation of mesenchymal stem cells (MSCs) from umbilical cord blood (UCB) from full-term deliveries is a laborious, time-consuming process that results in a low yield of cells. In this study we identified parameters that can be helpful for a successful isolation of UCB-MSCs. According to our findings, chances for a well succeeded isolation of these cells are higher when MSCs were isolated from UCB collected from normal full-term pregnancies that did not last over 37 weeks. Besides the duration of pregnancy, blood volume and storage period of the UCB should also be considered for a successful isolation of these cells. Here, we found that the ideal blood volume collected should be above 80 mL and the period of storage should not exceed 6 h. We characterized UCB-MSCs by morphologic, immunoph

SUBMITTER: Sibov TT 

PROVIDER: S-EPMC3432537 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature