TAT-mediated photochemical internalization results in cell killing by causing the release of calcium into the cytosol of cells.

ABSTRACT:

Background

Lysis of endocytic organelles is a necessary step in many cellular delivery methodologies. This is achieved efficiently in the photochemical internalization approach but the cell death that accompanies this process remains a problem.

Methods

We investigate the mechanisms of cell death that accompanies photochemical internalization of the fluorescent peptide TMR-TAT.

Results

TMR-TAT kills cells after endocytosis and light irradiation. The lysis of endocytic organelles by TMR-TAT causes a rapid increase in the concentration of calcium in the cytosol. TMR-TAT co-localizes with endocytic organelles containing calcium prior to irradiation and photochemical internalization leads to the release of the lumenal content of these organelles. Ruthenium red and cyclospo

SUBMITTER: Muthukrishnan N 

PROVIDER: S-EPMC3432654 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature