DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.

ABSTRACT:

Objectives

Cell lines derived from human ovarian and endometrial cancers, and their immortalized non-malignant counterparts, are critical tools to investigate and characterize molecular mechanisms underlying gynecologic tumorigenesis, and facilitate development of novel therapeutics. To determine the extent of misidentification, contamination and redundancy, with evident consequences for the validity of research based upon these models, we undertook a systematic analysis and cataloging of endometrial and ovarian cell lines.

Methods

Profiling of cell lines by analysis of DNA microsatellite short tandem repeats (STR), p53 nucleotide polymorphisms and microsatellite instability was performed.

Results

Fifty-one ovarian cancer lines were profiled with ten found to be redun

SUBMITTER: Korch C 

PROVIDER: S-EPMC3432677 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature