Polarity and asymmetry in the arrangement of dynein and related structures in the Chlamydomonas axoneme.

ABSTRACT: Understanding the molecular architecture of the flagellum is crucial to elucidate the bending mechanism produced by this complex organelle. The current known structure of the flagellum has not yet been fully correlated with the complex composition and localization of flagellar components. Using cryoelectron tomography and subtomogram averaging while distinguishing each one of the nine outer doublet microtubules, we systematically collected and reconstructed the three-dimensional structures in different regions of the Chlamydomonas flagellum. We visualized the radial and longitudinal differences in the flagellum. One doublet showed a distinct structure, whereas the other eight were similar but not identical to each other. In the proximal region, some dyneins were missing or replaced by mino

SUBMITTER: Bui KH 

PROVIDER: S-EPMC3432765 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature