Profiling of cytokines in human epithelial ovarian cancer ascites.

ABSTRACT:

Background

The behavior of tumor cells is influenced by the composition of the surrounding tumor environment. The importance of ascites in ovarian cancer (OC) progression is being increasingly recognized. The characterization of soluble factors in ascites is essential to understand how this environment affects OC progression. The development of cytokine arrays now allows simultaneous measurement of multiple cytokines per ascites using a single array.

Methods

We applied a multiplex cytokine array technology that simultaneously measures the level of 120 cytokines in ascites from 10 OC patients. The ascites concentration of a subset (n = 5) of cytokines that was elevated based on the multiplex array was validated by commercially available ELISA. The ascites level of these 5 cyt

SUBMITTER: Matte I 

PROVIDER: S-EPMC3433103 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature