Dynamic organization of transcription compartments is dependent on functional nuclear architecture.

ABSTRACT: Transcription in higher organisms requires spatiotemporal coordination of transcription machinery and the transcription factors at promoter sites. Toward this, recent evidence suggests that both static compartmentalization and dynamic self-organization of transcriptional apparatus are in effect at sites of transcription. Although the dynamics of transcription machinery is essential to genome regulation, the principles underlying this organization and its functional coupling to nuclear architecture is unclear. In a recent study we revealed that Uridine-5'-triphosphate (UTP) uptake in living cells labeled transcription-related compartments. In this article, we quantitatively establish multicolor labeling strategies for UTP-enriched transcription compartments (TCs) and probe their dynamic org

SUBMITTER: Maharana S 

PROVIDER: S-EPMC3433616 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature