Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules.

ABSTRACT: We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR. Dial-out PCR enables multiplex in vitro clone screening and is a compelling alternative to in vivo cloning and Sanger sequencing for accurate gene synthesis.

SUBMITTER: Schwartz JJ 

PROVIDER: S-EPMC3433648 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature