Cloning, characterization and expression analysis of a novel gene encoding Kunitz-type protease inhibitor from Dolichos biflorus

ABSTRACT: This paper reports the presence of a Kunitz-type protease inhibitor (HGPI) gene in Dolichos biflorus for the first time. A full-length protease inhibitor gene with complete open reading frame of 669 bp encoding 222 amino acids was cloned from D. biflorus using a PCR-based method. BlastN search showed that the HGPI gene shared 100% homology with Cicer arietinum trypsin inhibitor mRNA and 97% with Cajanus cajan protease inhibitor. The deduced amino acid sequence exhibited homology with Kunitz-type trypsin inhibitor from chickpea, pigeon pea and soybean. The deduced amino acid sequence contained N-terminal signal sequence of 18 amino acids and had a molecular weight of 24 kDa. The phylogenetic tree also showed close relationship with Cicer arietinum and Cajanus cajan. Southern blotting reveal

SUBMITTER: Kuhar K 

PROVIDER: S-EPMC3433878 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature