Developmental heterogeneity in DNA packaging patterns influences T-cell activation and transmigration.

ABSTRACT: Cellular differentiation programs are accompanied by large-scale changes in nuclear organization and gene expression. In this context, accompanying transitions in chromatin assembly that facilitates changes in gene expression and cell behavior in a developmental system are poorly understood. Here, we address this gap and map structural changes in chromatin organization during murine T-cell development, to describe an unusual heterogeneity in chromatin organization and associated functional correlates in T-cell lineage. Confocal imaging of DNA assembly in cells isolated from bone marrow, thymus and spleen reveal the emergence of heterogeneous patterns in DNA organization in mature T-cells following their exit from the thymus. The central DNA pattern dominated in immature precursor cells in

SUBMITTER: Gupta S 

PROVIDER: S-EPMC3434176 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature