YghG (GspSβ) is a novel pilot protein required for localization of the GspSβ type II secretion system secretin of enterotoxigenic Escherichia coli.

ABSTRACT: The enterotoxigenic Escherichia coli (ETEC) pathotype, characterized by the prototypical strain H10407, is a leading cause of morbidity and mortality in the developing world. A major virulence factor of ETEC is the type II secretion system (T2SS) responsible for secretion of the diarrheagenic heat-labile enterotoxin (LT). In this study, we have characterized the two type II secretion systems, designated alpha (T2SS(α)) and beta (T2SS(β)), encoded in the H10407 genome and describe the prevalence of both systems in other E. coli pathotypes. Under laboratory conditions, the T2SS(β) is assembled and functional in the secretion of LT into culture supernatant, whereas the T2SS(α) is not. Insertional inactivation of the three genes located upstream of gspC(β) (yghJ, pppA, and yghG) in the atypica

SUBMITTER: Strozen TG 

PROVIDER: S-EPMC3434588 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature