Cycling of CRYPTOCHROME proteins is not necessary for circadian-clock function in mammalian fibroblasts.

ABSTRACT:

Background

An interlocked transcriptional-translational feedback loop (TTFL) is thought to generate the mammalian circadian clockwork in both the central pacemaker residing in the hypothalamic suprachiasmatic nuclei and in peripheral tissues. The core circadian genes, including Period1 and Period2 (Per1 and Per2), Cryptochrome1 and Cryptochrome2 (Cry1 and Cry2), Bmal1, and Clock are indispensable components of this biological clockwork. The cycling of the PER and CRY clock proteins has been thought to be necessary to keep the mammalian clock ticking.

Results

We provide a novel cell-permeant protein approach for manipulating cryptochrome protein levels to evaluate the current transcription and translation feedback model of the circadian clockwork. Cell-permeant cryptochrome p

SUBMITTER: Fan Y 

PROVIDER: S-EPMC3434691 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature