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Efficient replication bypass of size-expanded DNA base pairs in bacterial cells.

ABSTRACT: Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.

SUBMITTER: Delaney JC 

PROVIDER: S-EPMC3434874 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Efficient replication bypass of size-expanded DNA base pairs in bacterial cells.

Delaney James C JC   Gao Jianmin J   Liu Haibo H   Shrivastav Nidhi N   Essigmann John M JM   Kool Eric T ET  

Angewandte Chemie (International ed. in English) 20090101 25

Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners. ...[more]

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