Real-time transcriptional profiling of cellular and viral gene expression during lytic cytomegalovirus infection.

ABSTRACT: During viral infections cellular gene expression is subject to rapid alterations induced by both viral and antiviral mechanisms. In this study, we applied metabolic labeling of newly transcribed RNA with 4-thiouridine (4sU-tagging) to dissect the real-time kinetics of cellular and viral transcriptional activity during lytic murine cytomegalovirus (MCMV) infection. Microarray profiling on newly transcribed RNA obtained at different times during the first six hours of MCMV infection revealed discrete functional clusters of cellular genes regulated with distinct kinetics at surprising temporal resolution. Immediately upon virus entry, a cluster of NF-κB- and interferon-regulated genes was induced. Rapid viral counter-regulation of this coincided with a very transient DNA-damage response, foll

SUBMITTER: Marcinowski L 

PROVIDER: S-EPMC3435240 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature