DNA methylation profiles of airway epithelial cells and PBMCs from healthy, atopic and asthmatic children.

ABSTRACT:

Background

Allergic inflammation is commonly observed in a number of conditions that are associated with atopy including asthma, eczema and rhinitis. However, the genetic, environmental or epigenetic factors involved in these conditions are likely to be different. Epigenetic modifications, such as DNA methylation, can be influenced by the environment and result in changes to gene expression.

Objectives

To characterize the DNA methylation pattern of airway epithelial cells (AECs) compared to peripheral blood mononuclear cells (PBMCs) and to discern differences in methylation within each cell type amongst healthy, atopic and asthmatic subjects.

Methods

PBMCs and AECs from bronchial brushings were obtained from children undergoing elective surgery for non-respiratory con

SUBMITTER: Stefanowicz D 

PROVIDER: S-EPMC3435400 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature