Chronic ingestion of alcohol modulates expression of ubiquitin editing enzyme A20 in lung macrophages.

ABSTRACT:

Background

Alcohol abuse is involved in the pathogenesis of multiple organ disorders; the underlying mechanism is incompletely understood. The ubiquitin editing enzyme A20 is involved in regulating activities in the cell. Suppression of A20 is suggested as one factor in the initiation of inflammation. This study investigates the mechanism by which chronic alcohol consumption modulates the levels of ubiquitin editing enzyme A20 in macrophages and further contributes to induce endothelial barrier dysfunction in the lung.

Methods

Mice were gavage-fed with 40% alcohol daily for 0-3 weeks. Airway macrophages were collected by lung lavage. Expression of ubiquitin editing enzyme A20 in isolated macrophages was assessed at both mRNA and protein levels. The endothelial barrier functi

SUBMITTER: Huang QY 

PROVIDER: S-EPMC3436623 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature