Distinct and contrasting transcription initiation patterns at Mycobacterium tuberculosis promoters.

ABSTRACT: Although sequencing of Mycobacterium tuberculosis genome lead to better understanding of transcription units and gene functions, interactions occurring during transcription initiation between RNA polymerase and promoters is yet to be elucidated. Different stages of transcription initiation include promoter specific binding of RNAP, isomerization, abortive initiation and promoter clearance. We have now analyzed these events with four promoters of M. tuberculosis viz. P(gyrB1), P(gyrR), P(rrnPCL1) and P(metU). The promoters differed from each other in their rates of open complex formation, decay, promoter clearance and abortive transcription. The equilibrium binding and kinetic studies of various steps revealed distinct rate limiting events for each of the promoter, which also differed marke

SUBMITTER: Tare P 

PROVIDER: S-EPMC3436766 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature