Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil.

ABSTRACT:

Background

Tuber magnatum, the Italian white truffle, is the most sought-after edible ectomycorrhizal mushroom. Previous studies report the difficulties of detecting its mycorrhizas and the widespread presence of its mycelium in natural production areas, suggesting that the soil mycelium could be a good indicator to evaluate its presence in the soil. In this study a specific real-time PCR assay using TaqMan chemistry was developed to detect and quantify T. magnatum in soil. This technique was then applied to four natural T. magnatum truffières located in different regions of Italy to validate the method under different environmental conditions.

Results

The primer/probe sets for the detection and quantification of T. magnatum were selected from the ITS rDNA regions. Their spe

SUBMITTER: Iotti M 

PROVIDER: S-EPMC3438110 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature