ELISA-mimic screen for synthetic polymer nanoparticles with high affinity to target proteins.

ABSTRACT: Synthetic polymer nanoparticles (NPs) that display high affinity to protein targets have significant potential for medical and biotechnological applications as protein capture agents or functional replacements of antibodies ("plastic antibodies"). In this study, we modified an immunological assay (enzyme-linked immunosorbent assay: ELISA) into a high-throughput screening method to select nanoparticles with high affinity to target proteins. Histone and fibrinogen were chosen as target proteins to demonstrate this concept. The selection process utilized a biotinylated NP library constructed with combinations of functional monomers. The screen identified NPs with distinctive functional group compositions that exhibited high affinity to either histone or fibrinogen. The variation of protein af

SUBMITTER: Yonamine Y 

PROVIDER: S-EPMC3438363 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature