Robust transformation procedure for the production of transgenic farmer-preferred cassava landraces.

ABSTRACT: Recent progress in cassava transformation has allowed the robust production of transgenic cassava even under suboptimal plant tissue culture conditions. The transformation protocol has so far been used mostly for the cassava model cultivar 60444 because of its good regeneration capacity of embryogenic tissues. However, for deployment and adoption of transgenic cassava in the field it is important to develop robust transformation methods for farmer- and industry-preferred landraces and cultivars. Because dynamics of multiplication and regeneration of embryogenic tissues differ between cassava genotypes, it was necessary to adapt the efficient cv. 60444 transformation protocol to genotypes that are more recalcitrant to transformation. Here we demonstrate that an improved cassava transformati

SUBMITTER: Zainuddin IM 

PROVIDER: S-EPMC3439245 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature