Genomic characterization of explant tumorgraft models derived from fresh patient tumor tissue.

ABSTRACT:

Background

There is resurgence within drug and biomarker development communities for the use of primary tumorgraft models as improved predictors of patient tumor response to novel therapeutic strategies. Despite perceived advantages over cell line derived xenograft models, there is limited data comparing the genotype and phenotype of tumorgrafts to the donor patient tumor, limiting the determination of molecular relevance of the tumorgraft model. This report directly compares the genomic characteristics of patient tumors and the derived tumorgraft models, including gene expression, and oncogenic mutation status.

Methods

Fresh tumor tissues from 182 cancer patients were implanted subcutaneously into immune-compromised mice for the development of primary patient tumorgraft mod

SUBMITTER: Monsma DJ 

PROVIDER: S-EPMC3439334 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature