Achieving efficient protein expression in Trichoderma reesei by using strong constitutive promoters.

ABSTRACT:

Backgrounds

The fungus Trichoderma reesei is an important workhorse for expression of homologous or heterologous genes, and the inducible cbh1 promoter is generally used. However, constitutive expression is more preferable in some cases than inducible expression that leads to production of unwanted cellulase components. In this work, constitutive promoters of T. reesei were screened and successfully used for high level homologous expression of xylanase II.

Results

The transcriptional profiles of 13 key genes that participate in glucose metabolism in T. reesei were analyzed by quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR). The results indicated that the mRNA levels of pdc (encoding pyruvate decarboxylase) and eno (encoding enolase) genes wer

SUBMITTER: Li J 

PROVIDER: S-EPMC3439336 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature