Organotypic brain slice cultures of adult transgenic P301S mice--a model for tauopathy studies.

ABSTRACT:

Background

Organotypic brain slice cultures represent an excellent compromise between single cell cultures and complete animal studies, in this way replacing and reducing the number of animal experiments. Organotypic brain slices are widely applied to model neuronal development and regeneration as well as neuronal pathology concerning stroke, epilepsy and Alzheimer's disease (AD). AD is characterized by two protein alterations, namely tau hyperphosphorylation and excessive amyloid β deposition, both causing microglia and astrocyte activation. Deposits of hyperphosphorylated tau, called neurofibrillary tangles (NFTs), surrounded by activated glia are modeled in transgenic mice, e.g. the tauopathy model P301S.

Methodology/principal findings

In this study we explore the benefit

SUBMITTER: Mewes A 

PROVIDER: S-EPMC3439393 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature