Spatially selective reagent delivery into cancer cells using a two-layer microfluidic culture system.

ABSTRACT: In this work, we demonstrate a two-layer microfluidic system capable of spatially selective delivery of drugs and other reagents under low shear stress. Loading occurs by hydrodynamically focusing a reagent stream over a particular region of the cell culture. The system consisted of a cell culture chamber and fluid flow channel, which were located in different layers to reduce shear stress on cells. Cells in the center of the culture chamber were exposed to parallel streams of laminar flow, which allowed fast changes to be made to the cellular environment. The shear force was reduced to 2.7 dyn cm(-2) in the two-layer device (vs. 6.0 dyn cm(-2) in a one-layer device). Cells in the side of the culture chamber were exposed to the side streams of buffer; the shear force was further reduced to

SUBMITTER: Liu Y 

PROVIDER: S-EPMC3439837 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature