DeltaPhage--a novel helper phage for high-valence pIX phagemid display.

ABSTRACT: Phage display has been instrumental in discovery of novel binding peptides and folded domains for the past two decades. We recently reported a novel pIX phagemid display system that is characterized by a strong preference for phagemid packaging combined with low display levels, two key features that support highly efficient affinity selection. However, high diversity in selected repertoires are intimately coupled to high display levels during initial selection rounds. To incorporate this additional feature into the pIX display system, we have developed a novel helper phage termed DeltaPhage that allows for high-valence display on pIX. This was obtained by inserting two amber mutations close to the pIX start codon, but after the pVII translational stop, conditionally inactivating the helper

SUBMITTER: Nilssen NR 

PROVIDER: S-EPMC3439877 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature