The generation of chromosomal deletions to provide extensive coverage and subdivision of the Drosophila melanogaster genome.

ABSTRACT:

Background

Chromosomal deletions are used extensively in Drosophila melanogaster genetics research. Deletion mapping is the primary method used for fine-scale gene localization. Effective and efficient deletion mapping requires both extensive genomic coverage and a high density of molecularly defined breakpoints across the genome.

Results

A large-scale resource development project at the Bloomington Drosophila Stock Center has improved the choice of deletions beyond that provided by previous projects. FLP-mediated recombination between FRT-bearing transposon insertions was used to generate deletions, because it is efficient and provides single-nucleotide resolution in planning deletion screens. The 793 deletions generated pushed coverage of the euchromatic genome to 98.4%. G

SUBMITTER: Cook RK 

PROVIDER: S-EPMC3439972 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature