Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.

ABSTRACT: We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

SUBMITTER: Hamady M 

PROVIDER: S-EPMC3439997 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature