Discovery of protein complexes with core-attachment structures from Tandem Affinity Purification (TAP) data.

ABSTRACT: Many cellular functions involve protein complexes that are formed by multiple interacting proteins. Tandem Affinity Purification (TAP) is a popular experimental method for detecting such multi-protein interactions. However, current computational methods that predict protein complexes from TAP data require converting the co-complex relationships in TAP data into binary interactions. The resulting pairwise protein-protein interaction (PPI) network is then mined for densely connected regions that are identified as putative protein complexes. Converting the TAP data into PPI data not only introduces errors but also loses useful information about the underlying multi-protein relationships that can be exploited to detect the internal organization (i.e., core-attachment structures) of protein com

SUBMITTER: Wu M 

PROVIDER: S-EPMC3440013 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature