Profiling environmental chemicals for activity in the antioxidant response element signaling pathway using a high throughput screening approach.

ABSTRACT:

Background

Oxidative stress has been implicated in the pathogenesis of a variety of diseases ranging from cancer to neurodegeneration, highlighting the need to identify chemicals that can induce this effect. The antioxidant response element (ARE) signaling pathway plays an important role in the amelioration of oxidative stress. Thus, assays that detect the up-regulation of this pathway could be useful for identifying chemicals that induce oxidative stress.

Objectives

We used cell-based reporter methods and informatics tools to efficiently screen a large collection of environmental chemicals and identify compounds that induce oxidative stress.

Methods

We utilized two cell-based ARE assay reporters, β-lactamase and luciferase, to screen a U.S. National Toxicology Progra

SUBMITTER: Shukla SJ 

PROVIDER: S-EPMC3440086 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature