Detection of prosecretory mitogen lacritin in nonprimate tears primarily as a C-terminal-like fragment.

ABSTRACT:

Purpose

Lacritin is a human tear glycoprotein that promotes basal tear protein secretion in cultured rat lacrimal acinar cells and proliferation of subconfluent human corneal epithelial cells. When topically added to rabbit eyes, lacritin promotes basal tearing. Despite these activities on several species, lacritin's presence in nonprimate tears or other tissues has not been explored. Here we probed for lacritin in normal horse tears.

Methods

Sequences were collected from the Ensembl genomic alignment of human LACRT gene with high-quality draft horse genome (EquCab2.0) and analyzed. Normal horse tears were collected and assayed by Western blotting, ELISA, and mass spectrometry. Newly generated rabbit antibodies, respectively, against N- and C-terminal regions of human lacrit

SUBMITTER: Laurie DE 

PROVIDER: S-EPMC3441044 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature