Widespread uncoupling between transcriptome and translatome variations after a stimulus in mammalian cells.

ABSTRACT:

Background

The classical view on eukaryotic gene expression proposes the scheme of a forward flow for which fluctuations in mRNA levels upon a stimulus contribute to determine variations in mRNA availability for translation. Here we address this issue by simultaneously profiling with microarrays the total mRNAs (the transcriptome) and the polysome-associated mRNAs (the translatome) after EGF treatment of human cells, and extending the analysis to other 19 different transcriptome/translatome comparisons in mammalian cells following different stimuli or undergoing cell programs.

Results

Triggering of the EGF pathway results in an early induction of transcriptome and translatome changes, but 90% of the significant variation is limited to the translatome and the degree of concor

SUBMITTER: Tebaldi T 

PROVIDER: S-EPMC3441405 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature