Comparison of microarray platforms for measuring differential microRNA expression in paired normal/cancer colon tissues.

ABSTRACT:

Background

Microarray technology applied to microRNA (miRNA) profiling is a promising tool in many research fields; nevertheless, independent studies characterizing the same pathology have often reported poorly overlapping results. miRNA analysis methods have only recently been systematically compared but only in few cases using clinical samples.

Methodology/principal findings

We investigated the inter-platform reproducibility of four miRNA microarray platforms (Agilent, Exiqon, Illumina, and Miltenyi), comparing nine paired tumor/normal colon tissues. The most concordant and selected discordant miRNAs were further studied by quantitative RT-PCR. Globally, a poor overlap among differentially expressed miRNAs identified by each platform was found. Nevertheless, for eight miRN

SUBMITTER: Callari M 

PROVIDER: S-EPMC3441572 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature