Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range.

ABSTRACT: Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.

SUBMITTER: Milles S 

PROVIDER: S-EPMC3441834 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature