Integration of environmental signals controls expression of Bordetella heme utilization genes.

ABSTRACT: The Bordetella pertussis heme utilization gene cluster hurIR bhuRSTUV encodes regulatory and transport functions required for assimilation of iron from heme and hemoproteins. Expression of the bhu genes is iron regulated and heme inducible. The putative extracytoplasmic function (ECF) sigma factor, HurI, is required for heme-responsive bhu gene expression. In this study, transcriptional activation of B. pertussis bhu genes in response to heme compounds was shown to be dose dependent and specific for heme; protoporphyrin IX and other heme structural analogs did not activate bhu gene expression. Two promoters controlling expression of the heme utilization genes were mapped by primer extension analysis. The hurI promoter showed similarity to sigma(70)-like promoters, and its transcriptional a

SUBMITTER: Vanderpool CK 

PROVIDER: S-EPMC344224 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature