Hydrophobic oxime ethers: a versatile class of pDNA and siRNA transfection lipids.

ABSTRACT: The manipulation of the cationic lipid structures to increase polynucleotide binding and delivery properties, while also minimizing associated cytotoxicity, has been a principal strategy for developing next-generation transfection agents. The polar (DNA binding) and hydrophobic domains of transfection lipids have been extensively studied; however, the linking domain comprising the substructure used to tether the polar and hydrophobic domains has attracted considerably less attention as an optimization variable. Here, we examine the use of an oxime ether as the linking domain. Hydrophobic oxime ethers were readily assembled via click chemistry by oximation of hydrophobic aldehydes using an aminooxy salt. A facile ligation reaction delivered the desired compounds with hydrophobic domain asym

SUBMITTER: Biswas S 

PROVIDER: S-EPMC3442363 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature