Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering.

ABSTRACT:

Background

2,3-Butanediol is a chemical compound of increasing interest due to its wide applications. It can be synthesized via mixed acid fermentation of pathogenic bacteria such as Enterobacter aerogenes and Klebsiella oxytoca. The non-pathogenic Saccharomyces cerevisiae possesses three different 2,3-butanediol biosynthetic pathways, but produces minute amount of 2,3-butanediol. Hence, we attempted to engineer S. cerevisiae strain to enhance 2,3-butanediol production.

Results

We first identified gene deletion strategy by performing in silico genome-scale metabolic analysis. Based on the best in silico strategy, in which disruption of alcohol dehydrogenase (ADH) pathway is required, we then constructed gene deletion mutant strains and performed batch cultivation of the stra

SUBMITTER: Ng CY 

PROVIDER: S-EPMC3442981 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature