High-throughput polymorphism detection and genotyping in Brassica napus using next-generation RAD sequencing.

ABSTRACT:

Background

The complex genome of rapeseed (Brassica napus) is not well understood despite the economic importance of the species. Good knowledge of sequence variation is needed for genetics approaches and breeding purposes. We used a diversity set of B. napus representing eight different germplasm types to sequence genome-wide distributed restriction-site associated DNA (RAD) fragments for polymorphism detection and genotyping.

Results

More than 113,000 RAD clusters with more than 20,000 single nucleotide polymorphisms (SNPs) and 125 insertions/deletions were detected and characterized. About one third of the RAD clusters and polymorphisms mapped to the Brassica rapa reference sequence. An even distribution of RAD clusters and polymorphisms was observed across the B. rapa ch

SUBMITTER: Bus A 

PROVIDER: S-EPMC3442993 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature