Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures.

ABSTRACT:

Background

The methylotrophic yeast Pichia pastoris has emerged as one of the most promising yeast hosts for the production of heterologous proteins. Mixed feeds of methanol and a multicarbon source instead of methanol as sole carbon source have been shown to improve product productivities and alleviate metabolic burden derived from protein production. Nevertheless, systematic quantitative studies on the relationships between the central metabolism and recombinant protein production in P. pastoris are still rather limited, particularly when growing this yeast on mixed carbon sources, thus hampering future metabolic network engineering strategies for improved protein production.

Results

The metabolic flux distribution in the central metabolism of P. pastoris growing on a mixe

SUBMITTER: Jorda J 

PROVIDER: S-EPMC3443025 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature