Expression of proto-oncogene KIT is up-regulated in subset of human meningiomas.

ABSTRACT:

Background

KIT is a proto-oncogene involved in diverse neoplastic processes. Aberrant kinase activity of the KIT receptor has been targeted by tyrosine kinase inhibitor (TKI) therapy in different neoplasias. In all the earlier studies, KIT expression was reported to be absent in meningiomas. However, we observed KIT mRNA expression in some meningioma cases. This prompted us to undertake its detailed analyses in meningioma tissues resected during 2008-2009.

Methods

Tumor tissues and matched peripheral blood samples collected from meningioma patients were used for detailed molecular analyses. KIT expression was ascertained immunohistochemically and validated by immunoblotting. KIT and KITLG transcript levels were discerned by reverse transcription quantitative real-time PCR (R

SUBMITTER: Saini M 

PROVIDER: S-EPMC3443037 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature