Efficient production of an engineered apoptin from chicken anemia virus in a recombinant E. coli for tumor therapeutic applications.

ABSTRACT:

Background

Apoptin, a nonstructural protein encoded by the VP3 gene of chicken anemia virus (CAV), has been shown to not only induce apoptosis when introduced into the precursors of chicken thymocytes, but has been found to specifically kill human cancer cells, tumor cell and transformed cells without affecting the proliferation of normal cells. This tumor-specific apoptotic characteristic of the protein potentially may allow the development of a protein drug that has applications in tumor therapy. However, several major problems, which include poor expression and poor protein solubility, have hampered the production of apoptin in bacteria.

Results

Significantly increased expression of recombinant full-length apoptin that originated from chicken anemia virus was demonstrated

SUBMITTER: Lee MS 

PROVIDER: S-EPMC3443062 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature