Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution.

ABSTRACT: Understanding translational control in gene expression relies on precise and comprehensive determination of translation initiation sites (TIS) across the entire transcriptome. The recently developed ribosome-profiling technique enables global translation analysis, providing a wealth of information about both the position and the density of ribosomes on mRNAs. Here we present an approach, global translation initiation sequencing, applying in parallel the ribosome E-site translation inhibitors lactimidomycin and cycloheximide to achieve simultaneous detection of both initiation and elongation events on a genome-wide scale. This approach provides a view of alternative translation initiation in mammalian cells with single-nucleotide resolution. Systemic analysis of TIS positions supports the r

SUBMITTER: Lee S 

PROVIDER: S-EPMC3443142 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature