A novel molecular signature for elevated tricuspid regurgitation velocity in sickle cell disease.

ABSTRACT:

Rationale

An increased tricuspid regurgitation jet velocity (TRV > 2.5 m/s) and pulmonary hypertension defined by right heart catheterization both independently confer increased mortality in sickle cell disease (SCD).

Objectives

We explored the usefulness of peripheral blood mononuclear cell-derived gene signatures as biomarkers for an elevated TRV in SCD.

Methods

Twenty-seven patients with SCD underwent echocardiography and peripheral blood mononuclear cell isolation for expression profiling and 112 patients with SCD were genotyped for single-nucleotide polymorphisms.

Measurements and main results

Genome-wide gene and miRNA expression profiles were correlated against TRV, yielding 631 transcripts and 12 miRNAs. Support vector machine analysis identified a 10-g

SUBMITTER: Desai AA 

PROVIDER: S-EPMC3443809 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature