Measurement of drug-protein dissociation rates by high-performance affinity chromatography and peak profiling.

ABSTRACT: The rate at which a drug or other small solute interacts with a protein is important in understanding the biological and pharmacokinetic behavior of these agents. One approach that has been developed for examining these rates involves the use of high-performance affinity chromatography (HPAC) and estimates of band-broadening through peak profiling. Previous work with this method has been based on a comparison of the statistical moments for a retained analyte versus nonretained species at a single, high flow rate to obtain information on stationary phase mass transfer. In this study an alternative approach was created that allows a broad range of flow rates to be used for examining solute-protein dissociation rates. Chromatographic theory was employed to derive equations that could be used

SUBMITTER: Schiel JE 

PROVIDER: S-EPMC3443976 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature