Transcriptional profiling of Caulobacter crescentus during growth on complex and minimal media.

ABSTRACT: Microarray analysis was used to examine gene expression in the freshwater oligotrophic bacterium Caulobacter crescentus during growth on three standard laboratory media, including peptone-yeast extract medium (PYE) and minimal salts medium with glucose or xylose as the carbon source. Nearly 400 genes (approximately 10% of the genome) varied significantly in expression between at least two of these media. The differentially expressed genes included many encoding transport systems, most notably diverse TonB-dependent outer membrane channels of unknown substrate specificity. Amino acid degradation pathways constituted the largest class of genes induced in PYE. In contrast, many of the genes upregulated in minimal media encoded enzymes for synthesis of amino acids, including incorporation of a

SUBMITTER: Hottes AK 

PROVIDER: S-EPMC344409 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature