TeiR, a LuxR-type transcription factor required for testosterone degradation in Comamonas testosteroni.

ABSTRACT: We have identified a new steroid-inducible gene (designated teiR [testosterone-inducible regulator]) in Comamonas testosteroni that is required for testosterone degradation. Nucleotide sequence analysis of teiR predicts a 391-amino-acid protein which shows homology between residues 327 and 380 (C-terminal domain) to the LuxR helix-turn-helix DNA binding domain and between residues 192 and 227 to the PAS sensor domain. This domain distribution resembles that described for TraR, a specific transcriptional regulator involved in quorum sensing in Agrobacterium tumefaciens. Analysis of the gene expression indicated that teiR is tightly controlled at the transcriptional level by the presence of testosterone in the culture medium. A teiR-disrupted mutant strain was completely unable to use testos

SUBMITTER: Pruneda-Paz JL 

PROVIDER: S-EPMC344414 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature