Culturable halophilic archaea at the initial and crystallization stages of salt production in a natural solar saltern of Goa, India.

ABSTRACT:

Background

Goa is a coastal state in India and salt making is being practiced for many years. This investigation aimed in determining the culturable haloarchaeal diversity during two different phases of salt production in a natural solar saltern of Ribandar, Goa. Water and sediment samples were collected from the saltern during pre-salt harvesting phase and salt harvesting phase. Salinity and pH of the sampling site was determined. Isolates were obtained by plating of the samples on complex and synthetic haloarchaeal media. Morphology of the isolates was determined using Gram staining and electron microscopy. Response of cells to distilled water was studied spectrophotometrically at 600nm. Molecular identification of the isolates was performed by sequencing the 16S rRNA.

Results

SUBMITTER: Mani K 

PROVIDER: S-EPMC3444409 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature